Methods in Molecular Biology Agrobacterium

This volume details Agrobacterium strain methods, such as plant genotype, transient and stable transformation of plants for gene overexpression, silencing, transplastomic and editing. Transformation protocols are focused mainly in plants with economic importance such as cereals and fruit trees including diploids and polyploids, and in non-plant species such fungi and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. This volume details Agrobacterium strain methods, such as plant genotype, transient and stable transformation of plants for gene overexpression, silencing, transplastomic and editing. Transformation protocols are focused mainly in plants with economic importance such as cereals and fruit trees including diploids and polyploids, and in non-plant species such fungi and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.