Animal Cell Culture Techniques

This manual on cell culture techniques addresses a variety of molecular and cellular biological questions, offering physiological conditions whilst avoiding using laboratory animals. In addition to basic techniques, a range of specialised practical protocols are included. Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. Cell culture techniques allow a variety of molecular and cellular biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a range of specialised practical protocols covering the following areas are included: cell proliferation and death; in-vitro models models for cell differentiation; in-vitro models for toxicology and pharmacology; industrial application of animal cell culture; and genetic manipulation and analysis of human and animal cells in culture.