Antibacterial activity of Lactobacillus spp against some pathogenic: bacteria isolated from burn infections in Al Najaf Ashraf

This study investigated the probiotic potential of Lactobacillus isolates from infants' feces and their antimicrobial activity against burn wound pathogens, particularly antibiotic-resistant Pseudomonas aeruginosa. 70 burn wound isolates from Al-Sadr Medical City and Al-Forat Hospital were identified, with P. aeruginosa (35.7%), S. aureus (25.7%), and K. pneumoniae (18%) most prevalent. Antibiotic resistance genes bla-TEM, bla-GES, bla-OXA, and bla-SHV were detected using PCR. 120 fecal samples from infants under 2 years yielded 57 Lactobacillus isolates, of which 10 showed strong antibacterial activity. All tolerated acid (pH 2.5) and bile (0.3%), with Isolate 10 showing 98.5% pH tolerance and 82% bile tolerance. MIC and MBC against P. aeruginosa were 256 µl/ml and 512 µl/ml, respectively. The isolates inhibited growth and biofilm formation of P. aeruginosa by 98.3%. 16S rRNA sequencing identified 5 L. acidophilus, 4 L. helveticus, and 1 Lactobacillus sp. These findings indicate Lactobacillus spp. could be a promising probiotic agent for controlling antibiotic-resistant burn wound infections.