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This study investigates the production and purification of lactic acid from raw milk using Lactobacillus acidophilus. It examines the physicochemical properties of milk, isolates and identifies lactic acid bacteria, and optimizes fermentation conditions. The research further explores downstream processing using ion exchange chromatography and analyzes protein profiles with SDS-PAGE. The findings contribute to efficient, cost-effective methods for industrial lactic acid production from dairy sources.
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