Methods in Molecular Biology Intracellular Lipid Transport

This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the book continues with in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoint, how lipid transfer proteins function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Intracellular Lipid Transport: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process. This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the book continues with in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoint, how lipid transfer proteins function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Intracellular Lipid Transport: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.