Methods in Molecular Biology Protocols for Gene Analysis

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Bol It is now twenty years since Cohen and Boyer's first steps into DNA cloning. The future of basic molecular biology must lie in the ability to analyze DNA (and especially the genes within it) starting at the DNA level. This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.

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It is now twenty years since Cohen and Boyer's first steps into DNA cloning. The future of basic molecular biology must lie in the ability to analyze DNA (and especially the genes within it) starting at the DNA level. This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.


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