Methods in Molecular Biology Retinal Ganglion Cells

This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellularfeatures of the central nervous system and the many traumatic and degenerative blinding diseases that affect them. This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellularfeatures of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.