Microbial β-Galactosidase Enzyme: Isolation, Purification and Characterization

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This book includs isolation and identification of some bacterial strains for the production of beta galactosidase enzyme (ß-gal).Selection of the most potent strain through testing different microbial cultures to produce extracellular ß-gal.Investigation of some fermentation parameters to maximize enzyme production by the selected strain using one variable at a time (OVAT) and statistical factorial design.Partial purification and stabilization of ß-gal by applying chemical modification and immobilization techniques.Characterization of the three forms of ß-gal (free, modified, and immobilized).Evaluation of thermal stability, kinetic and thermodynamic parameters of native, immobilized and modified enzymes.Study the transgalactosilation reaction of partial pure ß-gal and synthesis of galacto-oligosaccharides (GOS).Evaluation the prebiotic and anti-oxidant activities of the produced GOS using immobilized ß-gal.

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Pagina's: 306, Paperback, Eliva Press