Plant Tissue Culture for Horticultural Crops

Plant Tissue Culture for Horticultural Crops is a comprehensive reference designed for students, early-career researchers, and professionals in plant biotechnology. Compiled by experts at ICAR-Central Institute of Temperate Horticulture, it focuses on advanced in vitro techniques for temperate horticultural crops, especially apple (Malus × domestica). The book systematically covers methods such as somatic embryogenesis, anther culture for doubled haploids, genetic transformation, and protoplast fusion. It balances theoretical background with optimized lab protocols suitable for teaching and training. Routine lab operations like media preparation, sterilization, and acclimatization are well explained. Unique to this guide is its inclusion of emerging fields like nanotechnology, biosensors, and in vitro plant-microbe interactions. A dedicated chapter on troubleshooting enhances its real-world applicability. The book supports scalable and replicable methods tailored to the underrepresented temperate horticulture sector. It serves as both a technical manual and an innovation driver in areas such as crop improvement and germplasm conservation. Emphasis is placed on clean plant material production for sustainable horticulture. Designed for institutional use, it strengthens laboratory capacity and research culture. The content is suitable for use as a textbook, manual, or field reference. It promotes scientific rigor in propagation and biotechnology. Overall, the guide is a crucial tool for next-gen plant scientists.