Platelet Proteomics

The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. The groundbreaking new book that covers platelets, their role, and their study through proteomics research Platelets are defined as small anucleate cells that circulate in the blood and play a fundamental role in haemostasis and thrombosis. From a pharmacological perspective, unwanted platelet activation forms blood clots and promotes cardiovascular disease—the leading cause of death in the Western world. Platelet Proteomics focuses on the cutting-edge science in the emerging field of platelet proteomics research—a vital analytical method for the study of platelet activity. Written by a variety of contributors at the forefront of platelet research, this invaluable reference covers the recent developments in analyzing the proteome of basal and activating platelets in providing opportunities for advancing the discovery of new proteins that could eventually become drug targets for platelet-based illness. Features of this book include: Coverage of non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), and sub-proteome analyses (secretome/releasate, membrane proteins, organelles) A focus on the main research done on platelet proteomics, such as general proteome mapping, mapping signaling pathways, secretome and membrane proteomics, studies integrating other "-omics," and application to platelet-related diseases An examination of platelet-specific problems in a widely applicable fashion—the signaling proteome chapter serves as a model for investigations in many other cell types. Covering key principles as well as the latest applications in proteome analyses, Platelet Proteomics makes a significant contribution to an area of study with exciting potential. This inaugural foray into platelet exploration is a blueprint for the future—and is expansive enough in scope to reach across many scientifi c disciplines. The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.