Proteome Research: Two Dimensional Gel Electrophoresis and Identification Methods

Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements. Proteomics, a branch of functional genomics, can be defined as the large-scale analysis of proteins. In all these large-scale analysis processes there are three phases: (1) separation of the molecules to be analyzed; (2) characterization of the separated molecules; and (3) managing, handling and analysis of the data. This book is centered on the first two phases of proteomics, i.e. separation by two-dimensional electrophoresis and microcharacterization of the separated proteins. This choice is due to the fact that the data production phase is the major bottleneck of proteomics, while it is not the case in standard genomics. Emphasis has been placed on the rationale of each technique, so that the reader will be able to keep pace with the rapid evolution of the techniques. However, up-to-date protocols are also given to help the beginner in the field. Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.