The HPLC Survival Guide: Lab Detective’s Handbook

WHEN YOUR HPLC METHOD GOES WRONG, KNOW WHERE TO LOOK NEXT. A blank shows a peak. Pressure suddenly rises. Your peaks start tailing. Two compounds co-elute. Retention time drifts. >What do you check first? The HPLC Survival Guide: The Lab Detective's Handbook is a practical field manual for diagnosing and troubleshooting real-world reversed-phase HPLC problems in pharmaceutical QC and method development. Rather than focusing on academic theory alone, this guide uses a problem-first, diagnostic approach: recognize the symptom, investigate the likely causes, and determine the most appropriate next action. The book contains 14 field missions covering ghost peaks and baseline noise, backpressure, peak shape, stationary-phase selection, mobile-phase control, first-run method development, retention-time drift, resolution and co-elution, gradient problems, UV/DAD detection, calibration and system suitability, peak integration, sample and injection effects, and method transfer and robustness. >Whether you are developing a method, troubleshooting a failed run, investigating an unexpected chromatogram, or working through a QC laboratory problem, this guide is designed to help you move from "What happened?" to "What should I check next?" Stop guessing. Start diagnosing. The HPLC Survival Guide: The Lab Detective's Handbook Mirela Gorjanu Practical Pharma Science(TM)